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- ItemA spartial framework for managing sewer and water networks using sensor networks: a case of the university of Zambia.(The University of Zambia, 2023) Chibuye, GabrielPublic institutions generally have a lot of facilities and infrastructure catering for the population of the institution. Some important infrastructure such utilities which include water, sewer, electricity, and telecommunications require constant maintenance, management, and adequate monitoring for them to function effectively. This study aimed to build a framework for Managing Sewer and Water Networks Using Sensor Networks at the University of Zambia. The methodology involved understating the existing operations of the Resident engineer’s office to ascertain the user needs. These needs formed the basis of the study, the development and design of the prototype of the Web based Framework. Data Collection was done using various methods. A presurvey (Reconnaissance Survey) was done to have an idea of the existing infrastructure and a more accurate survey was done using GPS GNSS equipment GIS software was used to process data and create shapefiles. A 15-digit coding system was created and was used to assign unique identification numbers and extra attribute information to the water and sewer network shapefiles. A QR code was generated for each node and the serial number recorded. Tagging was done using the QR code for each structure. All the shapefiles were loaded into a PostGreSQL spatial database and were used as input in the Web GIS application. The web application had some tools imbedded in it which were mainly for monitoring and management of the structures. Using internet of things (IOT) technology, water level sensors were placed in a prototype to mimic some critical manholes to help with the monitoring of the sewerage network. The system could therefore be used for identification of any infrastructure by scanning the QR code and a map showing the structure would be seen online by the field technicians. Students could also be able to send out notifications to the resident engineer in case there was a damaged or malfunctioning structure. Monitoring of the sewer system could be done using the sensors and ultimately the framework would ease the management of the water and sewer systems at the University of Zambia.
- ItemA pilot study to identify single nucleotide variants in exon 19 of the braca1 gene in female breast cancer patients at Matero level one hospital, Lusaka, Zambia.(The University of Zambia, 2022) Shakachite, LisaBreast cancer (BC) patients in Zambia more commonly present with advanced-stage disease and often at young ages (≤50 years). Breast cancer gene 1 (BRCA1) is a tumor suppressor gene in which mutations have been linked to disease presentation at a young age. More than 600 distinct pathogenic (disease causing) single nucleotide variants with the potential for structural and functional effects on BRCA1 have been documented in ClinVar, a major human variation and phenotype database. The BRCA1 C-terminal (BRCT) region is critical for tumor suppression because it acts in DNA damage repair and cell cycle checkpoint protein control. Mutations in this region have been associated to structural effects and loss of function of BRCA1 in many studies. However, BRCT mutations in young female breast cancer patients from Zambia have not been identified to date. The genetic alterations in exon 19 of the BRCA1 gene were assessed here for the first time in a cohort from Zambia. Seventy-three patients diagnosed with breast cancer at the age of ≤50 years were recruited irrespective of family history of breast and/or ovarian cancer. Genomic DNA was extracted from ten peripheral blood samples and PCR-amplified with exon 19-targeting primers of the BRCA1 gene. Amplicons were sequenced using a Sanger sequencing platform and analyzed using bioinformatics tools at various web-based platforms and ClinVar. Overall, elevensingle nucleotide mutations were identified in three patients, and all known to play a role in determining the structure of BRCA1. Of the identified mutations, one was pathogenic and sevenhad uncertain significances. Six of these mutations were heterozygous and classified in the loss of function category in ClinVar. These variants were specifically associated with Breast-ovarian cancer, familial, susceptibility to, 1 (BROVCA1). Four out of the six heterozygous mutations were missense mutations and two were nonsense mutations. Based on an unpaired two-tailed student’s t-test, the null hypothesis that there was no association between the age of a BC patient and the number of mutations in exon 19 leading to a loss of function of BRCA1, was rejected (P= 0.04). Further studies are clearly necessary to explore how mutations in the entire BRCA1 gene affect disease presentation in the female BC population. The young age (≤50 years) for breast cancer diagnosis in female Zambian patients may be attributed to other factors like other genetic mutation status, epigenetic changes, lifestyle etc. These results will help in decision making with regard to the integration of genomic medicine into breast cancer care in Zambia which has tremendous potential to improve the outcomes and survival of diagnosed patients.
- ItemAbudance,growth and reproductive biology of oreochromis niloticus (Linneaus,1758)compared with tilapiines indigenous to the middle Zambezi(The University of Zambia, 2017) Nyirenda, Steinslaus MasuzyoThe introduction of Oreochromis niloticus on Lake Kariba has been widely associated with the decline in preponderance of indigenous Tilapiines, especially Oreochromis mortimeri. Comperative aspects of abundances, growth and reproductive biology for O. niloticus, and three known indigeous Tilapiines: Oreochromis andersonii, Oreochromis mortimeri, and Tilapia rendalli were investigated. The study was conducted at the riparian Sinazongwe District of Southern Zambia from August to September, 2014. Weight, length and reproductive stages of the different Tilapiine fish were determined. The morphometric and age data was used to determine growth parameters for the Tilapiines using Von Bertalanffy Growth Function (VBGF). Whole ovaries from sexually mature females (ripe and/ or ripe-running) were weighed and preserved in 5% formalin solution; fecundity was determined using gravimetric procedures. Analyses of Variance (ANOVA) were applied in comparing statistical significances in abundance, growth and reproductive parameters among the studied Tilapiines. Detailed contribution by weight and number for each Tilapiine species at Sinazongwe indicated dominance of O. niloticus (IRI=8.62%) whereas T. rendalli accounted for 0.77%. Oreochromis andersonii and O. mortimeri made insignificant IRI contributions (<1%). Investigation into dependence of Tilapiine abundance on intrinsic growth rate, (K) indicated a positive linear relationship defined by a statistically insignificant regression equation: Abundance=92.49K-8.39 (R2 =0.94; p>0.05). Specimens collected ranged in age from 2-6 years. The intrinsic growth rate, K, was highest in T. rendalli (0.791yr-1), followed by O. mortimeri (0.308yr-1), O. niloticus (0.180yr-1) and least in O. andersonii (0.147yr-1). Growth performance indices (Φ') for the species differed marginally, being highest for T. rendalli (4.98) and O. niloticus (4.58), and moderately lower in O. mortimeri (4.57) and O. andersonii (4.36). There were significant differences (p<0.05) observed on the fecundities among the studied female Tilapiines. Tilapia rendalli had highest average number of oocytes, 5,135. Among the Oreochromis species, O. niloticus had an average 2,923 oocytes whereas O. andersonii had 1,650 oocytes. On the other hand, O. mortimeri had the lowest fecundity, 994 oocytes. Oreochromis niloticus was the most abundant Tilapiine at Sinazongwe, and indeed an established species throughout Lake Kariba, on the Zambian shoreline. The studied characteristics among the Tilapiines indicated that O. niloticus was not superior in every aspect of its growth and reproductive biology.
- ItemAcaricide resistance in Rhipicephalus appendiculatus Neumann, Amblyomma Variegatum (Fabricius) and Boophilus decoloratus (Koch) in Southern and Central Provinces of Zambia(2012-08-20) Luguru, Stephen M. K.JAcaricide resistance tests were conducted on 14-21 days old larvae of Rhipicephalus appendiculatus Neumann, Amblyomma variegatum (Fabricius) and Boophilus decoloratus (Koch), using the Food and Agriculture Organization (FAO) Acaricide Resistance Test Kit, and acaricide test papers prepared locally according to the FAO method. Engorged female ticks for larval production were collected from different localities in the Southern and Central (including Lusaka) Provinces of Zambia. The lowest LC50(%) values obtained from sets of data for each tick species were used to calculate resistance factors (RF).The range of LC50(%) values for R. appendiculatus and for B. decoloratus in brackets using the FAO Kit were: cypermethrin, 0.025-0.30 (0.03-0.045), coumaphos, 0.13-0.60, dioxathion, 0.20-0.60 (0.35-0.60), diazinon, 0.03-0.13 (0.015-0.06) and dieldrin, 0.08-0.30. For local papers values were: cypermethrin, 0.026-0.260 (0.012-0.056), chlorfenvinphos, 0.02-0.08 (0.012-0.12), dioxathion, 0.062-0.47 (0.03-0.50) and deltamethrin, 0.005-0.015 (0.004-0.011). For A. variegatu/n values with the FAO Kit and values with local papers in brackets were: dioxathion, 0.15-0.26 (0.032-0.32), cypermethrin, 0.03-0.082 (0.01-0.084), dieldrin, 0.05-0.32 and chlorfenvinphos (0.011-0.052). These results indicated that cattle ticks in the Southern and Central Provinces of Zambia are developing resistance to acaricides. B. decoloratus in the commercial sector and R. appendiculatus in the traditional sector showed resistance to a number of acaricides whereas Amblyomma variegatum showed resistance to dieldrin and dioxathion. Boophilus decoloratus from the traditional sector were however, relatively susceptible. The observed pattern of resistance is attributed to a number of factors such as the frequency of acaricide application, cattle management and type of cattle and other agricultural and ecological factors. Although cypermethrin and dioxathion were the only acaricides which were both in the FAO Kit and locally prepared test papers, results obtained showed some agreements between the two types of test papers. From the results, it is possible to identify resistance using local papers on the same lines as would be with the FAO papers. Since chemical control of ticks is the most practical method of controlling ticks and tick-borne diseases in Zambia, the usage of acaricides should be carefully monitored to avoid development of multiple resistance in tick populations. Although locally prepared papers have their limitations, it is important to note that they may be a useful tool in enabling economically poor countries like Zambia to detect early resistance in ticks in the absence of the standardised FAO Resistance Test Kit. An integrated approach to tick control involving less use of acaricides is discussed.
- ItemAdaptation of Trypanosoma (NANNOMONAS) Congolense broden 1904 types to different hosts and transmission by Glossina species(2012-09-19) Masaninga, FreddieThe influence of host bLood on midgut infection, maturation rates in Glossina species, transmission and virulence to mice of Trypanosoma congolense DNA types was investigated. The study was also coiKiucted to identify an optimum technique for isolating Nannomonas stocks from the field. Trypanosoma congolense parasites used in this study were identified by the Polymerase Chain Reaction Technique. Zebra and goat blood supported higher midgut and labral infection rates of T. congolense in Glossina morsitans centralis than pig, donkey, black rhinoceros, Thomson's gazelle and eland blood. Of these hosts, zebra supported the h^est metacyclic loads in the hypopharynx. The proportion of midgut infections maturing (transmission index) was generally high for most blood types. It ranged from about 58.0% for tsetse infected with eland blood to 97.0% for tsetse infected with zebra blood. From a practical view point, this study suggests that areas with many goats should have higher infection rates of some T. congolense genotypes. Zebra being a less favoured host probably has a lesser inpact on the infection rates. The pattern of T. congolense Savannah, Kilifi and Riverine / Forest infection rates in G. m. centralis infect«l with goat, black rhinoceros and Boran cow blood was similar during the first 10 days of infection. Rates were highest (up to 90%) on day 3, and dropped 1^ day 5 to reach constant values (ranging from 5% to 45%) by day 10. The 10th day midgut infection rates did not differ significantly from those on day 21 in fifes infected with pig, zebra, goat and eland blood. Carbohydrate binding proteins (lectins) and otter factors are considered to play a role in controlling trypanosoma establishment and maturation. Male G. m. centralis infected with zebra and eland blood matured significantly more gut infections than females. A higher proportion of mice became infected with T. congolense when the infection was initiated by the bite of male tsetse (56.0%) than when the infection was initiated by the bite of a female tsetse (33.0%). This firming was interpreted to suggest an inportant impact on the application of the Sterile Male Technique (SMT) for controlling tsetse flies. Kilifi T. congolense took a significantly shorter tin« to infect mice (13 days) than the Savannah DNA type (18 days). The Kilifi type of T. congolense (K60/1) killed a higher proportion of mice than the Savannah type within 45 days after infection. It also showed earlier parasitaemic peaks than the Savannah type, thus suggesting that the former (Kilifi T. congolense) used in this investigation is nwre virulent than the latter (Savannah T. congolense). Studies on parasite diversity have implications on trypanosomiasis control because they can provide clues to questions on factors that maintain trypanosomes at endemic or factors that can encourage trypanosomes to go into epidemic levels. Trypanosoma congolense (Kilifi type) was detected earlier in mice when the transmitting tsetse was fed on infective goat blood (mean =11 days) than When they were fed on infective eland blood (mean = 14 days). Transmission rates of Kilifi and Savannah types of T. congolense to mice were similar and were not influenced by the host blood type used to infect the transmitting fly. Therefore, the study suggests that host blood can exert host-specific effects on metacyclic devetopment in the tsetse fly that can be subsequently observed when the animal are transmitted with the trypanosomes. Isolation and cloning of trypanosomes by mouse isolation were more effective in Balb/c than Swiss mice. Isolation of T. congolense through gut-form transfer fi-om one tsetse species to another (procyclic expansion technique) was most effective in laboratory reared G. m. centralis and not effective in G. pallidipes and G. m. morsitans. Tsetse passaged with procyclics suspended in fresh goat blood generally survived well during the observation period (survival rates ranged from 58% to 95%). Cholesterol and D(+)-glucosamine added to the goat infective bloodmeal increased infection rates of son» but not all stocks of procyclics. Double cholesterol feeds supported significantly higter midgut infection rates than single cholesterol feeds. Tsetse fed on cholesterol-trypanosome-blood mixtures survived well (survival rates ranged from about 60% to 80%). D(+)-glucosamine facilitated infection rates of procyclics in G. m. centralis but also caused mortality. Pocyclics incubated in different blood mixtures, including cow and goat serum, reconstituted goat red blood cells, heated or unheated, retailed viability for a day at room temperature (25°C.). This suggested that the media used for cryopreserving procyclics was not a critical factor. This study suggests that it is feasible to isolate T. congolense types of trypanosomes by feeding laboratory - reared G. m. centralis tsetse on fresh goat blood and chemical additives such as cholesterol, but maintaining the flies on a rabbit. This finding is discussed in relation to other methods such as the use of G. m. morsitans fed on synthestic diets or the combined use of culture and laboratory reared tsetse flies to isolate T. congolense from wild hosts.
- ItemAflatoxin Metabolites in Relation to Protein-Energy Malnutrition in Zambia(2011-04-05) Samuel, Derina SaraFindings in the Sudan show a possible association between aflatoxins and kwashiorkor, a category of Protein-Energy Malnutrition (PEM). Similarities in the geographical and seasonal prevalence of aflatoxins and kwashiorkor, as well as similarities in the metabolic derangements caused by aflatoxins and observed in kwashiorkor suggest that aflatoxins may play an etiological role in the pathogenesis of kwashiorkor. Studies in Zambia have shown that aflatoxin contamination occurs in commonly eaten foods, suggesting that the Zambian population is exposed to the toxin. The presence of urinary aflatoxins in patient with and without liver pathology at an incidence which reflects the level of contamination has also been reported. PEM is known to be endemic in Zambia witlva seasonal prevalence similar to that observed with aflatoxin contamination of food. A one-year survey was therefore undertaken to investigate the relationship between aflatoxins and the various categories of PEM. Metabolism of the aflatoxins was determined by analyzing urine and serum samples of PEM cases and appropriate controls from the University Teaching Hospital, Lusaka. As previous studies had shown a higher level of aflatoxin contamination in foods in the Eastern province, urine samples from PEM cases and appropriate controls were obtained from St. Francis1 Hospital, Katete, for comparison. The incidence of urinary aflatoxins in samples from Lusaka and Katete was 6.1 per cent, with a mean concentration of 0.365 ng/ml. Aflatoxin B, and aflatoxicol were detected. Aflatoxicol concentrations were highest in control and lowest in kwashiorkor samples, though aflatoxicol was detected slightly more frequently in the latter. Aflatoxin B, excretion was observed to follow a similar pattern. Comparing the incidences in Lusaka and Katete, a significant difference was not observed. In serum samples the incidence of aflatoxins was 2.8 per cent, with a mean concentration of 1.87 ng/ml. Aflatoxin B, and aflatoxicol were detected with aflatoxicol only being detected in one case of kwashiorkor. Total aflatoxin concentration was higher in kwashiorkor than controls while the incidence was similar in the two groups. The incidence of urinary aflatoxin in the rainy season (18.8 per cent) was greater than in the dry season (5 per cent), however, no significant difference was observed in the seasonal variation of aflatoxins in sera. Urinary aflatoxins were detected more often in males than in females but no significant difference occurred in the sera analyses. The results suggest that there may be certain unusual associations in the incidence, concentrations and type of aflatoxin detected between the different nutritional groups, in particular the role of aflatoxicol in kwashiorkor and the lower excretion levels observed. Possibly this indicates a decreased capacity to deal with aflatoxins. Whether this a cause or an effect of the kwashiorkor syndrome, is still not clear. These preliminary findings merit an extended study to examine more closely the relationship of aflatoxins and kwashiorkor in Zambian children.
- ItemAFlatoxin residues in edible tissue of poultry in Zambia(2011-04-05) Nalube, Sidney MuntangaThe aflatoxins, toxins produced by the fungus Aspergillus flavus, have been detected in both imported as well as locally grown maize in Zambia.The problem of toxicity caused by the ingestion of aflatoxin-contaminated maize was approached by determining the levels of residues in edible tissue of poultry from known dietary levels of aflatoxin in maize. The livers and spleens, gizzards and hearts, and thighs were chosen for aflatoxin residue analysis. This study was conducted to determine the aflatoxin residue levels in tissues of village chickens that had been fed diets containing total aflatoxin concentration of 4750mg/kg of feed (equivalent to aflatoxin Bl concentration of 1650 mg/kg) at 0% (for controls) 25% (for low AF diet) and 75% (for high AF diets) inclusion levels for 21 days. Residues of aflatoxin were determined by thin layer chromatography. Results show that aflatoxins Bl, B2,Gl, G2 and Ml were deposited in all tissues analysed. The highest levels were present in the livers and spleens (8.0 mg/kg, low AF diet; 15.4 mg/kg, high AF diet) and the lowest were in the thighs (1.6 mg/kg, low AF diet; 9.4 mg/kg,high AF diet) . At the end of the experiment, the livers were observed to be pale, enlarged and friable.Since only a small fraction (less than 1%) of the highest level of aflatoxin tested and ingested was deposited in the tissues, either as the original compound or as its metabolite, there appears to be little danger of carry-over of dietary aflatoxin to poultry tissue. The toxic levels (i.e. levels that can cause death) to humans is considered to be approximately 1.7 mg/kg daily (Campbell and Stoloff, 1974), which means that the amount the poultry would need to consume to reach this tissue level would cause pathological symptoms or even death. Therefore the chance of these contaminated birds entering the food chain can be considered to be minimal since people traditionally do not eat poultry dying of unknown cause.
- ItemAgricultural transformation in zambia’s Chibombo, Kapiri Mposhi and Mumbwa districts, during the periods 1980-1990 and 1997-2008(University of Zambia, 2019) Malambo, Augrey HicigaaliThe governance philosophies of the UNIP and MMD governments were very different. These were expected to drastically influence economic performance and livelihoods. The study was undertaken to compare how agricultural crop production among smallholder farmers changed in time and space. The main objective of this study was to establish the patterns of smallholder crop farming and agricultural transformation of Chibombo, Kapiri Mposhi and Mumbwa districts in Central Province of Zambia between the1980-1990 and 1997-2008 periods. An institutional theoretical framework and a household conceptual approach were used to guide the conduct of this study at the institutional and household levels. The neoliberal theory framework of the 1990s was the basis of the emergence of post-1997 agricultural institutions while the dependency theory of the 1980s guided the agricultural institutions of the 1980s. The Institutional theory guided analysis of institutional changes while the Household Economic Approach was used to analyze effects of policies on families. The establishment of changes which had taken place in smallholder crop farming after 1997 and emerging transformations justified the study. Secondary data was obtained through an extensive review of literature. Primary data was obtained through questionnaires distributed to various agricultural officers and 1,367 smallholder farmers in 226 cooperatives. Other pieces of primary data were obtained through Group and personal interviews and observations. Maps were used in the comparison of agricultural patterns existing between 1980-1990 and 1997-2008 periods. Data was analyzed using quantitative and qualitative methods. The study established that the main crops grown during the 1980-1990 period included maize, cotton and groundnuts. After 1997 only maize and cotton continued to dominate while solanum macrocarpon (impwa), sweet potatoes and other crops emerged. The mono cropping system of the 1980s gave way to multicropping, crop rotation and conservation farming practices. Furthermore, after 1997 maize and cotton production remained relatively stable while production of other crops not prominent during the 1980-1990 period increased. Agricultural support institutions of the 1980s collapsed and their places were taken by new privately owned and more sustainable but less spatially distributed institutions. The quality of transport infrastructure deteriorated while two short roads were tarred in Chibombo District. Liberalization became more entrenched especially around market centres and in easily accessible areas of the study districts. In parts of farming areas where government improved and/or up-scaled its policy implementation, sustainable, rewarding and growing agricultural development emerged while in areas where policies were not well implemented or were not followed or not followed correctly, only marginal and declining agricultural development occurred. It is concluded that maize and cotton were widely grown in both study periods; Sunflower production declined after 1997 while new crops such as solanum macrocarpon became common after 1997. The government supported agricultural institutions of the 1980-1990 period collapsed at the end of the 1980s and, new privately and more sustainable institutions emerged. The state of road infrastructure declined after 1997 while positive agricultural transformations characteristic of liberalization emerged near accessible farming areas and market centres. The study concluded that after 1997 changes occurred in cropping systems, the type of crops they grew and crop production; agricultural support institutions; transport infrastructure and new transformations emerged. Key Words: Agricultural Liberalization, Agricultural Support Institutions, Agricultural Transformation, Farmer Response, Smallholder Crop Farming, Transport Infrastructure.
- ItemAgro Informatics System(2015-10-05) Velemu, CharlesAgriculture can be defined as the science or practice of farming, including the growing of crops and the rearing of animals.Agriculture is one of the most important sectors in Zambia's economy and could benefittremendously with the application of information communication technology. Due to tremendous advances during the last decade, information technology is today affecting all the spheres of human life. We can exploit these advances to design a cost-effective system to provide expert advice to farmers and the general public.This project focuses on how information communication technology can be used to better improveagriculture in Zambia.This will be done by implementing an online electronic Agro trading system, a livestock andvegetable farming information repository, a google maps implementation showing cropdistribution in different farming areas of Zambia and an electronic records management system forlocal cooperatives and associations.
- ItemAn evaluation of tourism sector participation in the Livingstone city integrated development plan 2005- 2010, Zambia.(The University of Zambia, 2022) Zulu, MaxwellIntegrated development planning is a process that requires the involvement of stakeholders in determining the strategic development priorities to their development needs. Stakeholder participation is an instrument used meeting citizen’s development needs when they influence and share control over their development. Besides, stakeholder participation provides opportunities for stakeholders to have access to decision-making processes that inform and shape the formulation and implementation of integrated development plans (IDPs). Stakeholder participation is one of the fundamental principles in the formulation and implementation of IDPs.The first City of Livingstone IDP was a five-year plan targeted for implementation between 2005 and 2010. The plan’s strategic focus was tourism development and stakeholder participation from the tourism sector was key in the development of the IDP because of the tourism focus of the IDP. Their engagement was perceived to be cardinal in shaping the Livingstone City IDP and its implementation, which in the long run, influence the promotion of tourism in the City of Livingstone.This study aimed at assessing the extent to which stakeholders in the tourism sector in the City of Livingstone influenced the formulation and implementation of the Livingstone City IDP. The study utilised a case study method which focused more on qualitative data generated from interviews and observations. The sampling approach of the respondent was purposive sampling, and 27 respondents were interviewed. The collected data were analysed qualitatively using themes that emerged from the responses in the field research.The findings revealed that the key stakeholders in the tourism industry in the study area did not adequately participate in the formulation of the Livingstone IDP. The stakeholder participation mechanisms used did not yield meaningful stakeholder engagement. It led to stakeholders in the tourism sector not being influential during the formulation and implementation of the plan.The study concluded that most of the key stakeholders did not participate in the formulation of the first Livingstone IDP (2005-2010) primarily focused on tourism development and hence, its inability to be effectively implemented. This conclusion demonstrates that stakeholder participation is very critical in the entire process of formulation and implementation of the IDPs in Zambia.
- ItemAn analysis of public perceptions of domestic solid waste management: The case of the make Zambia clean and healthy programme in Livingstone(2015-04-13) Chilinga, GiversSolid Waste Management (SWM) continues to be a major concern in Zambia. As such, the Government of the Republic of Zambia (GRZ), through the Ministry of Local Government and Housing (MLGH) in 2004 instituted the Make Zambia Clean and Healthy (MZCH) campaign aimed, in part, at enhancing solid waste collection and disposal to designated dumpsites countrywide. Therefore, a survey on public perceptions of Domestic Solid Waste Management (DSWM) was, between the March and April, 2013, conducted to evaluate the effectiveness of the MZCH campaign in the city of Livingstone. Three representative localities of the city, presumably of different socioeconomic status (with low, medium and high density households), were selected and surveyed. Although the study was largely quantitative, an eclectic approach was used to collect and analyse field data. A total of 202 respondents were successfully sampled through combined simple random and systematic methods. Coded data from the 202 respondents were entered into the SPSS version 16.0 to generate descriptive statistics, and application of Multivariate Analysis of Variance (MANOVA). MANOVA results show that only two independent variables (monthly income and residence classification) reached statistical significant multivariate differences at p ≤ 0.05. For the monthly income variable, F (6, 376) = 3.55, p=0.002; Wilks’ Lambda (λ) = 0.89; partial eta squared = 0.054; and residence classification, F (6,392) = 2.09, p=0.05; Wilks’ Lambda (λ) = 0.94; partial eta squared = 0.031. This MANOVA result indicates firstly that the demographic factors do not influence public perceptions of the effectiveness of the DSWM system under the MZCH programme. Secondly, there were no major differences in public perceptions of the DSWM system among the different socioeconomic households of the city of Livingstone. Further, the results of this study suggest that the campaign was generally viewed as being ineffective, as local community members largely felt they did not participate in the decision making and implementation of the programme. Overall, the findings of this study suggest that the goals of the campaign were not in tandem with the local community’s preferred DSWM strategies. There is a need, therefore, to involve local community members in identifying future waste management solutions and to provide information to all concerned persons about practical aspects of waste management.
- ItemAn analysis of the functional characterisitcs and potential of periodic markets (Minada) in Mbala district(University of Zambia, 2016) Nyirenda, Kasonde ChilumbaA market is a physical place where goods and services are bought and sold, with buyers and sellers coming in contact with one another. They are classified according to their spatial and temporal specialisation, thus categorised as daily, periodic and special markets. Periodic markets happen once on fixed days of each week or month in smaller market centres which are insufficient to merit permanence. Four markets were selected and surveyed in Mbala District. Sampling of the markets was done by stratified and simple random methods. Respondents (traders) were selected using simple random sampling and judgmental sampling for buyers. The key informants were traditional leaders and local council officials. An eclectic approach was used to collect and analyse field data. Coded data was entered into Excel 2007 to generate descriptive statistics. This study was conducted to analyse the distribution, functions and potential of periodic markets in Mbala District. Market meetings were organised in their own spatial and temporal arrangement in order to achieve optimal benefits. Periodic markets in Mbala performed socio-economic roles; as such they are multi-functional institutions. Economically, they were points of exchange for local goods and services; points of collection, bulking and distributions of various goods and services; they created employment; provided alternative places for trading and the MAFF used them to monitor market prices for agriculture commodities. Periodic markets also performed some political and social functions. There effects included increased returns for the trader and taking various manufactured goods closer to the buyers. However, problems such as excessive beer drinking, sexual immorality, thefts and a threat to local food security were noticeable. Regression Analysis was used to test the two formulated hypotheses, results indicated that most traders regularly visited other periodic markets to sale and or buy because it was more rewarding. Patronage was negatively affected by distance and age; the farther the distance of a market centre and the older a person grew, the lesser was patronage. Recommendations included the rehabilitation and maintenance of feeder roads for the free flow of goods and services; to improve sanitary conditions in markets; to assist some traders with financial loans to increase sustainability; to regulate and discourage trade that threatened local food security, and to conduct further research and studies on periodic markets to understand them more.
- ItemAnalysis of the mycotoxins of fusarium(2012-08-28) Siame, Anthony BupeFusarium contamination of maize has been shown to be a problem in Zambia. In this study it was decided to test for the presence of five toxins produced by Fusarium; Zearalenone, T-2 toxin, diacetoxyscirpenol (DAS), deoxynivalenol (DON) and nivalenol, in maize and maize products. This was the first major study involving the analysis of trichothecenes in Zambia, and thus several methods of extraction and determination of the fo,ur tricho-thenes and Zearalenone in maize and mixed feeds were tested, in order to arrive at the most suitable methods for use in our Laboratory. The methods finally adopted gave 80, 80,86, 50 and 60 per cent recoveries of added Zearalenone, DON, nivalenol, T-2 toxin and DAS respectively. TLC was used for qualitative as well as quantitative determination of the toxins and as low as 50 (jg/kg Zearalenone, 80 |jg/kg DON and nivalenol, and about 2000 pg/kg T-2 toxin and DAS could be determined. Mouldy maize, rejected by NAMBOARD but mostly used by farmers as animal feed supplement or in the brewing of local opaque beer, was collected from farmers around Lusaka and analysed for the five Fusarium toxins. This maize was found to contain only Zearalenone and DON in the range of 0.08-6.0 mg/kg and 0.5-16 mg/kg respectively. A year long survey of mixed animal feeds from National Milling Company was initiated and 148 samples were analysed for Zearalenone, DON and nivalenol. 17 per cent of the samp¬les contained Zearalenone (0.05-0.6 mg/kg) and 1.4 per cent of the samples contained DON (1.0 mg/kg). No nivalenol was detected in any of the samples. The Zearalenone and DON positive samples were further tested for T-2 toxin and DAS but none was detected. This is the first report of the natural occurrence of Zearalenone and DON in mixed feed samples in Zambia. An isolate of F. graminearum was gro.wn at 26 C and 16°C corresponding to average maximum and minimum temperatures often recorded near the end of the rainy season. Zearalenone production was found to be favoured by low temperatures (16 C) while DON production was found to be favoured by high temperatures (26 C ).
- ItemAnalysis of the nutrient content of some indegenous Zambian food plants with some studies of processing and storage(2013-02-03) Mainga, Aaron MelekiIn this study, six indigenous Zambian food plants have been analysed for their nutrient content. These include two leafy wild vegetables, two wild fruits and two wild root tubers. The leafy vegetables were also dried and stored under traditional storage conditions and later analysed for their nutritive value. Two processed foods, have also been studied and subjected to nutrient analysis. The nutrition factors examined are moisture, ash, crude fat, crude protein, carbohy¬drate plus fibre, energy, beta-carotene, vitamin C and the minerals phosphorus, calcium, iron and zinc. The purpose of this study was to provide essential biochemical data on the nutrient content of some widely consumed Zambian food plants. The data is necessary for accurate and quantitative evaluation of dietary and clinical data by Zambian nutritionists and medical personnel. The data is also necessary for the compilation of Zambian food composition tables by the National Food and Nutrition Commission, a governmental agency that co-ordinates nutrition matters. A comparison of the results obtained in this study to those obtained elsewhere has been discussed. Frottt the results oJ>tained it has been found that the two fresh leafy vegetables are good sources of beta-carctene and vitamin C, These also contain fair amounts of proteins and minerals. Dry stored vegetables have been ^otind to retain their nutritive value except for vitamin C. The two fruits have been found to be good sources cf vitamin C, carbohydrates, energy and minerals. The two root tubers were found to be good sources of carbohydrates and energy. On the processed foods, the dough has been found to be rich in fat, protein and phosphorus. The fruit beverage has been found to contain all the nutrients present in the fruit except they are present in smaller quantities due to dilution.
- ItemAntischistosomal structure-activity relationships of nitrated n-phenyl benzamide derivatives.(The University of Zambia, 2023) Sichinga, JohnSchistosomiasis is a chronic parasitic disease caused by blood-dwelling trematodes of the genus Schistosoma. It ranks second on a list of neglected tropical parasitic diseases. Globally, about 280,000 deaths are recorded annually while more than 240 million people get infected and more than 700 million people risk being infected. The disease is associated with approximately 3.3 million disability-adjusted life years. It also causes a total estimated annual global economic loss amounting to US$641,790,130. Africa alone accounts for more than 90% of schistosomiasis cases with Schistosoma mansoni and Schistosoma haematobium species being the most prevalent. For over 40 years, praziquantel has stood out as the principal drug of choice in the treatment and control of schistosomiasis. It is efficacious and safe. It has also been very instrumental in preventive chemotherapy. However, it faces emerging drug resistance, lacks antischistosomal activity against juvenile worms, does not provide a 100% cure, and does not prevent re-infection. N-phenylbenzamides have shown potential as antischistosomal agents although they have not been extensively studied. This study was inspired by one compound, MMV687807, which was active against Schistosoma mansoniworms and was 100% lethal at 10 µM against Schistosoma haematobium adult worms whose further medicinal chemistry exploration, identified MK1-11, from the recent study that reported its exhibited potency against Schistosoma mansoni adult worms attributed to trifluoromethyl group (CF3), an electron-withdrawing group. This study, therefore, sought to further enhance activity by nitrating the core-scaffold of the front-runner compound, MK1-11.The analogs of this compound were thus synthesized and tested in vitro on Schistosoma mansoni juvenile worms. The successfully synthesized analogs were confirmed by HPLC-MS, 1H-NMR, and 13C-NMR spectroscopic techniques. The 1H-NMR and 13C-NMR results were analyzed using a spectral data analyzing software called MestReNova. The %-death-of-worms effect produced by the tested compounds were 62%, 28%, 56%, and 42% at 50 µM with reduced effects of 41.67%, 25%, 54.17%, and 25% at 10 µM respectively. Structure-activity relationships (SARs) appeared to confirm earlier findings being electron-withdrawing groups are essential for potency. Generally, analogs containing nitro and trifluoromethyl groups on the phenyl rings as depicted in JS-03 and JS-05 exhibited enhanced activity. However, regioisomerism (ortho or meta substitution) in these two analogs did not seem to affect activity. Due to low activity on juvenile worms, the analogs could not be tested on adult worms. Consequently, enhancement or decrease in activity with respect to the front-runner compound,MK1-11 which was only tested on adult worms and also using a different in vitro biological assay method could not be deduced. There is need, therefore, to consider resynthesizing the front-runner compound by further research, test it on both juvenile and adult worms using the same in vitro assay method as the newly synthesized analogs in order to assess to what extent activity is improved upon or compromised.
- ItemAn appraisal of household management in the city of Chipata(The University of Zambia, 2019) Namonje, Blessing TwavweThe purpose of the study was to carry out an appraisal of the management of household waste in the city of Chipata. The study was based on the following objectives, to: (i) characterise household waste in Chipata city, in terms of composition, volume, recyclability and reusability, and (ii) examine the management strategies of household waste carried out in Chipata city. A survey design supported by both qualitative and quantitative approaches was used and by using a checklist, questionnaire and interview guide relevant information on household waste composition, storage, handling and disposal were obtained from selected households of Kapata, Hollywood and Kalongwezi Townships of Chipata city. A sample of 120 households was involved. Stratified sampling was used to obtain the three townships so that high, medium and low density populated residential areas were represented while purposive sampling was applied to select 40 households per township. Quantitative data was analysed using descriptive statistics with the aid of the Statistical Software Package for Social Sciences 20.0 (SPSS) while qualitative data was coded and emerging themes were grouped into categories and then interpreted. The results showed a marked variation in domestic waste types and quantity generated across the three residential areas reflecting the income levels of the inhabitants. The highest total weights (26.2 kilograms) were generated in Kalongwezi Township, area with low population while the lowest values (18.0 Kilograms) were reported in the highly populated Kapata area showing that despite some wealthier areas having much lower populations than poorer areas, they still generate more waste. In Kapata Township, plastic bags were the common receptacles used and more than 50 percent of the households had no receptacles meaning that open dumping was prevalent. Burning and burying of waste was observed in Kapata and Hollywood where waste dumping in drains and waste littering characterized by overfilled receptacles was also common. The waste generated in these townships contained less reusable materials compared to Kalongwezi where more reusable waste was generated. The results of this study, therefore, provide evidence that many households in Chipata do not efficiently manage their household waste. The study recommended the following; reenforcement of the existing legal framework in the country, improving public awareness through sensitization programmes, encouraging the minimisation of waste generation, introducing incentives to those who separate waste and encouraging private agencies to participate in both waste recovery and in general waste management efforts. Building on this study, research could be undertaken to assess the generation of household waste in all districts in the country as well as the household waste composition and storage facilities. Key Words: Household Waste, Waste Management, Indiscriminate Waste Disposal, Receptacle
- ItemAssessing shopping mall led urban restructuring in Lusaka: location factors and key actors.(2023) Musonda, AbishaiThe aim of the research was to examine the factors that determine the location of shopping malls and the resulting urban restructuring that has taken place in Lusaka. It was guided by three objectives; to evaluate the factors that determine the location of shopping malls; to identify the key actors in the location of shopping malls; and to assess the urban restructuring that has taken place. Interview guide was used to collect data from 28 shopping mall owners and 3 officials from LCC, ZEMA and MLG purposively selected based on their job description, experience and knowledge on the research topic. The resulting data was categorized, tabulated and analysed using content analysis. In terms of factors that determine the Location of shopping malls in Lusaka, the study identified five factors; plot size, access to major roads, market area, demographics and distance between shopping malls. The study established that these factors are well known by the developers and not the institutions that are mandated to regulate the process. In relation to actors, the study identified the following; the developers, ZEMA, LCC and MLG as the main stakeholders in the location of shopping malls. The findings further revealed that most shopping malls are driven by the developers who lack planning professional expertise. The developers have high interest and high power to drive the process of location of shopping malls. Their interest is making sure that they get approval of the potential site they have identified with or without following the planning regulations. With regard to the urban restructuring, the study finds that twenty-eight sites that were formerly used as small shops (such as hardware, mechanical workshop and grocery), Greenfields (playground, burial site, vegetation cover) and police post/council offices have been replaced by shopping malls. These shopping malls have changed the aesthetic beauty of the sites. The study further established that most of the urban restructuring has been done on sites that were initially earmarked for small shops. In conclusion, the study has revealed that the location of shopping malls in Lusaka is been driven by developers to suit their interests. The Ministry of Local Government and Lusaka city council is only managed these decisions administratively. In addition, location of shopping malls are products of inadequate legislation, weak enforcement and lack of shopping mall policy. Therefore, the study recommends that the enforcements of the existing laws should be strengthened by ZEMA, LCC and MLG. In addition, a shopping mall policy that will fully regulate the location of shopping mall should be developed by MLG. This would help decision makers to address the social, economic and environmental concerns associated with shopping malls.
- ItemAn assessment of Animal draught power in agricultural production: A case of Matondo Village-Kalomo District(2015-06-15) Hamoonga, Webster L.M.The general objective of this study was to make an assessment of the role of animal draught power and the diffusion of donkey farm technology in agricultural production. The focus of the study were the oxen and the donkey owners. The target was to see how donkey farm mechanization is spreading and its effectiveness in agriculture production. The present agriculture production and pre-ECF epidemic period agriculture production were also investigated.Generally, animal draught power plays a key role in the development of the agricultural sector. This is due to its capability to increase the agriculture productivity. However, animal draught power particularly oxen has been affected by East Coast Fever (ECF) disease resulting in deaths of hundreds of cattle. This has seen a reduction in agricultural productivity and high levels of poverty. Therefore, one potential substitute to oxen has been identified as donkeys. Hence, the donkey farm power is believed to be an alternative farm power that will reduce the country's dependence on cattle that are more vulnerable to many diseases. Therefore, to carry out this research, the households were chosen randomly and out of the 60 households, 40 households were sampled. Hence, the method used to get this sample was the judgmental or purposive method. This was due to the nature of the village with unstable and mobile household members. As a result, those households which were viewed as typical representative of the sample or researcher's interest were chosen.The study therefore revealed that the current agriculture production is hot as it was before the pre-ECF epidemic period. There is low production due to fewer oxen used in agriculture production. On the other hand, some slight improvement in crop production in Matondo Village has been witnessed although not more than pre- ECF period. This increase in the crop productivity is attributed to a number of people who have adopted the donkey farm mechanization as an alternative farm power. As a result, the adoption of donkey farm mechanization has helped to increase crop productivity of Matondo Village as well as Kalomo District. Therefore, the study revealed that there is a relationship between animal draught power and agriculture production. This means, households with animal draught power have the capacity to produce enough with some surplus for both home consumption and for sale. Further, it was found that the recent recovery in agriculture production is a result of the recent diffusion of donkey farm technology in Matondo Village. To test these hypothesis to find out whether there is any relationship between the used variables, the Spearmann's Rank Correlation was used. In addition, the study revealed that although some households have adopted the donkeys, the rate of diffusion is very slow. This is due to its low status in the village. As a result, some measures were suggested by the villagers such as giving donkey loans or capital to buy donkeys, teaching fanners the effectiveness of the donkeys in agriculture production, giving small scale farmers free donkeys and launching of some sensitization campaigns to boost the status of donkeys.Finally, the study revealed some problems farmers face in the agriculture production. However, more importantly, the study suggested measures that can help to ensure even adoption of the donkeys as an alternative fann power such as linking them to cheaper sources of donkeys.
- ItemAn assessment of enchroachment and resource management of Luano National Forest, Chingola(2015-05-20) Mwila, Roselyne MLuano national forest has come under a lot of pressure from people venturing into agriculture, charcoal production over exploitation of timber and encroachment after retrenchment from the mines. Lack of forest management has led to environmental degradation and hinders socio-economic development.The questionnaire interview method was employed in obtaining information from stakeholders and the local people. The information helped in evaluating the management strategies employed in Luano national forest. Personal interviews and direct observation were conducted to get the qualitative information that could not be captured through other means of data collection. To determine the extent of encroachment, a satellite image for August, 2000 and digitized analogue maps for 1994 and 1995 were overlayed to get the land cover map.The study findings revealed that Luano national forest is heavily encroached with a high level of anthropogenic activities. The local people do not view the area as the forest but they consider it as an agricultural area. Of the 50 people interviewed, 90 percent were males and 10 percent were females. 64 percent of the respondents live in Luano area while 30 percent and 6 percent of the respondents live in Chingola and Kitwe, respectively. Poverty was cited as the main reason for the people to go and settle in the forest as it accounted for 72 percent of the answers obtained. Fertile soils and a place to settle accounted for 22 percent and 6 percent, respectively. The factors depleting resources in Luano forest were identified as charcoal production, conversion of the forest to agriculture land, encroachment, settlement, fire, and poverty.Lack of monitoring processes from the understaffed forest department contributed to encroachment of the forest. The forest officials concentrate on the management of local forests where Joint Forest Management strategies are employed. Even though the companies utilizing resources from the forest are ready to contribute to the sustainable management of the forest, there are no management strategies being employed in the forest.Luano forest should be degazeted so that it becomes a joint management entity for sustainable utilization of natural resources.
- ItemAn assessment of heavy metal contamination of groundwater in George compound of Lusaka(The University of Zambia, 2017) Nambeye, AliceThis study investigated groundwater contamination in a suburban area of George Compound located on the outskirts of the city of Lusaka in Zambia. The aim was to assess heavy metal contamination of groundwater and the local people’s awareness about the contamination in the study area. The objectives were fivefold; to (i) ascertain the usage of groundwater from wells among residents of George Compound; (ii) determine the concentration of heavy metals (cadmium, chromium, copper, iron and lead) in groundwater of the sampled wells in George Compound; (iii) examine the extent to which industries in close proximity to George Compound contributed to heavy metal contamination; (iv) find out local people’s level of awareness and knowledge about heavy metal contamination of the groundwater; and (v) find out whether or not past sensitisation campaigns have had an impact on the local people’s level of awareness of groundwater contamination in George Compound. A case study design was employed to ensure a detailed understanding of the situation. The data were collected through interviews, questionnaire, field observations and water quality measurements. Purposive and snowball sampling procedures were used in the selection of a sample of 38 respondents. Water sampling in wet and dry seasons was conducted on 14 purposively sampled wells. Respondents included 14 households with wells and 24 households without. Additionally, four (4) key informants were interviewed. Analysis of data revealed that, in the dry season, there were high levels of chromium (Cr) (0.14 mg/l), cadmium (Cd) (0.91 mg/l), and iron (Fe) (1.22 mg/l) as maximum concentrations above the Zambia Bureau of Standards (ZABS) for drinking water. Conversely, lead (Pb) and copper (Cu) concentration were found to be below the ZABS’s permissible limits. For the wet season, parameters found to have concentrations above the ZABS limits were chromium (0.64 mg/l), cadmium (0.32 mg/l), iron (3.11 mg/l), and lead (0.07 mg/l), while copper (Cu) (0.04 mg/l) was below the limit. The sources of these heavy metals were attributed to the manufacturing industries and illegal waste sites. A total of 45 percent of the sampled residents in George Compound were unaware of the groundwater contamination. It is concluded that groundwater in shallow wells in George Compound is contaminated with heavy metals (cadmium, chromium, iron and lead). This calls for regular groundwater quality monitoring by Water Resources Management Authority (WARMA) and Zambia Environmental Management Agency (ZEMA) for evidence to support strict enforcement of compliance by industries on discharge of effluents that meet ZABS standards in order to reduce health risks among residents.