A study of Enzyme variation and chloroquine sensitivity of plasmodium falciparum in Vitro
Date
2011-04-01
Authors
Lemnge, Moshi Moses Martha
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Abstract
Blood sampler- from children with malaria, were used
to set up iri_ vitro cultures of Plasmodium falciparam using
fresh human serum to enrich the culture medium. A number
of investigation:-; airafid at improving the i_n vitro cultivation
t e c h n i q u e ware carried out. Changing culture; medium two times
every 24 hours instead of once had n positive effect on the
final parasitemia level. Any e f f e c t of extra glucose on
parasite growth was not obvious within the two asexual cycles
period considered. A number of human sera treated at 56
58 C for 30-45 minutes to Qestrojr complement resulted in a
parasitemia twice that obtained with ordinary sera. A
further observation on change of so rum was made on a
continuous culture which had been maintained in two different
human sera for the first 37 days in vitro. The cultures
where serum was changed displayed a marked reduction in
parasitemia though this was only temporary. However, when
heat treated seruir was used no decrease in parasitemia was
apparent.
S tarch-ge 1-e loc trophoreses ol dirf^r-^nt isolates of
P. ralcj^parum fron Zambia revealed enzyme " •=> n i 3 t-j o n in r-i nr o.c"
phosphate isornerase ( G P I ) , 6-phospho gluconate denydrogenase
( 6 - P G D ) , and adenosine dean/inase ( A D A ) .
Parasite lactato d-.ihydrogens.se (L DH) and peptidase-E (PEP-E)
showed no v a r i a t i o n . /- rere enzyme form, 5-PGD-2, was
observed at a frequency of 80% but a standard is required
to c o n f i rm the e n z y ;-ie typ n ,
Five different isnlatos of F. fai_c_i.p:aj^urR wci'-e subjected
to different low c ore tivt r-at i ons of chloroquina ijri vitro,.
Their grovrth was s r c c o s r fully inliibi to d by this drug and
they were classifiad as b::ing sensitive. Parasite growth
was narkedly reduced after 48 hours of drug administration
and completely inhibited by 72 hours although for the last
2U hours culture was in drug-free medium.
Description
Keywords
Enzymes.