Protection by Nigella sativa against carbon tetrachloride-induced downregulation of hepatic cytochrome P450 isozymes in rats
Date
2008
Authors
Muzandu, Kaampwe
Ibrahim, Zein S.
Ishizuka, Mayumi
Soliman, Mohamed
ElBohi, Khlood
Wageh, Sobhy
Elkattawy, Azza M.
Sakamoto, Kentaro Q.
Fujita, Shoichi
Journal Title
Journal ISSN
Volume Title
Publisher
Japanese Journal of Veterinary Research
Abstract
Nigella sativa (family Ranunculaceae) is an annual plant that has been traditionally used on
the Indian subcontinent and in Middle Eastern countries. In this study, we investigated the effect
of N. sativa oil on the drug-metabolizing cytochrome P450 (CYP) enzymes and whether it
has a protective effect against the acute hepatotoxicity of CCl4. Intraperitoneal injection of
rats with CCl4 drastically decreased CYP2E1, CYP2B, CYP3A2, CYP2C11, and CYP1A2 mRNA
and protein expressions. Oral administration of 1 ml/kg N. sativa oil every day for one week
prior to CCl4 injection alleviated CCl4-induced suppression of CYP2B, CYP3A2, CYP2C11, and
CYP1A2. Moreover, CCl4 increased iNOS and TNFα mRNA, while N. sativa oil administration
for one week prior to CCl4 injection down regulated the CCl4-induced iNOS mRNA and upregulated
IL-10 mRNA. These results indicate that N. sativa oil administration has a protective
effect against the CCl4-mediated suppression of hepatic CYPs and that this protective effect is
partly due to the down regulation of NO production and up-regulation of the anti-inflammatory
IL-10.
Description
Journal article
Keywords
Carbon tetrachloride, , Cytochrome P450 , Nigella sativa oil