• Login
    View Item 
    •   UNZA Repository Home
    • Veterinary Medicine
    • Disease Control
    • View Item
    •   UNZA Repository Home
    • Veterinary Medicine
    • Disease Control
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Detection of Salmonella invA by isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) in Zambia

    Thumbnail
    View/Open
    Article (137.3Kb)
    Date
    2005-09-13
    Author
    Yabe, John
    Isogai, Emiko
    Makungu, Chitwambi
    Sinkala, Patson
    Nambota, Andrew
    Isogai, Hiroshi
    Fukushi, Hideto
    Silungwe, Manda
    Mubita, Charles
    Syakalima, Michelo
    Hang’ombe, Bernard Mudenda
    Kozaki, Shunji
    Yasuda, Jun
    Type
    Article
    Language
    en
    Metadata
    Show full item record

    Abstract
    The isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) is a new isothermal DNA amplification method composed of exo Bca DNA polymerase, RNaseH and DNA–RNA chimeric primers. We detected invA of Salmonella from chicken carcasses, egg yolk and cattle fecal samples. Fifty-three of 59 isolates were invA-positive in ICAN-chromatostrip detection. The result was consistent with those obtained by standard PCR. Salmonella invA was detected in 12 of 14 carcass rinses by ICAN, while in 7 of 14 rinses by standard PCR. These results indicate that ICAN is an efficient, sensitive and simple system to detect invA of Salmonella species in developing countries such as Zambia.
    URI
    http://dspace.unza.zm/handle/123456789/5790
    Publisher
    Elsevier
    Subject
    Salmonella
    invA
    ICAN--PCR--Zambia
    Description
    Journal article
    Collections
    • Disease Control [10]

    DSpace software copyright © 2002-2016  DuraSpace
    UNZA homepage | UNZA Library | Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of UNZA RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    DSpace software copyright © 2002-2016  DuraSpace
    UNZA homepage | UNZA Library | Contact Us | Send Feedback
    Theme by 
    Atmire NV