Detection of Salmonella invA by isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) in Zambia
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Date
2005-09-13
Authors
Yabe, John
Isogai, Emiko
Makungu, Chitwambi
Sinkala, Patson
Nambota, Andrew
Isogai, Hiroshi
Fukushi, Hideto
Silungwe, Manda
Mubita, Charles
Syakalima, Michelo
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) is a new
isothermal DNA amplification method composed of exo Bca DNA polymerase, RNaseH and
DNA–RNA chimeric primers. We detected invA of Salmonella from chicken carcasses, egg yolk and
cattle fecal samples. Fifty-three of 59 isolates were invA-positive in ICAN-chromatostrip detection.
The result was consistent with those obtained by standard PCR. Salmonella invA was detected in
12 of 14 carcass rinses by ICAN, while in 7 of 14 rinses by standard PCR. These results indicate that
ICAN is an efficient, sensitive and simple system to detect invA of Salmonella species in developing
countries such as Zambia.
Description
Journal article
Keywords
Salmonella , invA , ICAN--PCR--Zambia