Detection of Salmonella invA by isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) in Zambia

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Date
2005-09-13
Authors
Yabe, John
Isogai, Emiko
Makungu, Chitwambi
Sinkala, Patson
Nambota, Andrew
Isogai, Hiroshi
Fukushi, Hideto
Silungwe, Manda
Mubita, Charles
Syakalima, Michelo
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Volume Title
Publisher
Elsevier
Abstract
The isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) is a new isothermal DNA amplification method composed of exo Bca DNA polymerase, RNaseH and DNA–RNA chimeric primers. We detected invA of Salmonella from chicken carcasses, egg yolk and cattle fecal samples. Fifty-three of 59 isolates were invA-positive in ICAN-chromatostrip detection. The result was consistent with those obtained by standard PCR. Salmonella invA was detected in 12 of 14 carcass rinses by ICAN, while in 7 of 14 rinses by standard PCR. These results indicate that ICAN is an efficient, sensitive and simple system to detect invA of Salmonella species in developing countries such as Zambia.
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Keywords
Salmonella , invA , ICAN--PCR--Zambia
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