Protection by Nigella sativa against carbon tetrachloride-induced downregulation of hepatic cytochrome P450 isozymes in rats

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dc.contributor.authorMuzandu, Kaampwe
dc.contributor.authorIbrahim, Zein S.
dc.contributor.authorIshizuka, Mayumi
dc.contributor.authorSoliman, Mohamed
dc.contributor.authorElBohi, Khlood
dc.contributor.authorWageh, Sobhy
dc.contributor.authorElkattawy, Azza M.
dc.contributor.authorSakamoto, Kentaro Q.
dc.contributor.authorFujita, Shoichi
dc.date.accessioned2019-02-20T05:16:15Z
dc.date.available2019-02-20T05:16:15Z
dc.date.issued2008
dc.descriptionJournal articleen
dc.description.abstractNigella sativa (family Ranunculaceae) is an annual plant that has been traditionally used on the Indian subcontinent and in Middle Eastern countries. In this study, we investigated the effect of N. sativa oil on the drug-metabolizing cytochrome P450 (CYP) enzymes and whether it has a protective effect against the acute hepatotoxicity of CCl4. Intraperitoneal injection of rats with CCl4 drastically decreased CYP2E1, CYP2B, CYP3A2, CYP2C11, and CYP1A2 mRNA and protein expressions. Oral administration of 1 ml/kg N. sativa oil every day for one week prior to CCl4 injection alleviated CCl4-induced suppression of CYP2B, CYP3A2, CYP2C11, and CYP1A2. Moreover, CCl4 increased iNOS and TNFα mRNA, while N. sativa oil administration for one week prior to CCl4 injection down regulated the CCl4-induced iNOS mRNA and upregulated IL-10 mRNA. These results indicate that N. sativa oil administration has a protective effect against the CCl4-mediated suppression of hepatic CYPs and that this protective effect is partly due to the down regulation of NO production and up-regulation of the anti-inflammatory IL-10.en
dc.identifier.issn119-128
dc.identifier.urihttp://dspace.unza.zm/handle/123456789/5765
dc.language.isoenen
dc.publisherJapanese Journal of Veterinary Researchen
dc.subjectCarbon tetrachloride,en
dc.subjectCytochrome P450en
dc.subjectNigella sativa oilen
dc.titleProtection by Nigella sativa against carbon tetrachloride-induced downregulation of hepatic cytochrome P450 isozymes in ratsen
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