Genetic dissection of resistance to anthracnose in yellow bean collection of common beans (Phaseolus vulgaris L.).
| dc.contributor.author | Kuwabo, Kuwabo | |
| dc.date.accessioned | 2024-09-25T08:57:31Z | |
| dc.date.available | 2024-09-25T08:57:31Z | |
| dc.date.issued | 2023 | |
| dc.description | Thesis of Master of Science in Plant Breeding and Seed Systems. | |
| dc.description.abstract | Anthracnose caused by Colletotrichum lindemuthianum is a major disease of common bean (Phaseolus vulgaris) worldwide. Yellow beans are a major market class of common bean especially in eastern and southern Africa. The objectives of this study were i) evaluate the yellow bean collection for resistance to eight races of C. lindemuthianum, and ii) conduct genome-wide association analysis to identify genomic regions and candidate genes associated with resistance to eight races of C. lindemuthianum. The Yellow Bean Collection comprised of 255 diverse yellow bean genotypes was evaluated for resistance to races 5, 19, 39, 51, 81, 183, 1050 and 1105 of C. lindemuthianum. The Yellow Bean Collection was genotyped with 72, 866 SNPs using Genotyping by Sequencing and genome-wide association analysis was conducted using Mixed Linear Model in TASSEL. Several genotypes with superior levels of resistance to the eight races used in the current study were identified. The yellow bean genotype YBC278 was the only one among 255 genotypes that was highly resistant to all eight races. Resistance in the Yellow Bean Collection to the eight races used in the current study was controlled by major-effect loci on chromosomes Pv01, Pv03, Pv04, Pv05 and Pv07. The genomic region on Pv01, which overlapped with the Andean locus Co-1 provided resistance to races 81, 1050 and 1105. Significant SNPs for resistance to race 39 were identified on Pv02. The genomic region on Pv04, which overlaps with known major-effect loci Co-3, Co-15, Co 16, Co-y and Co-z, provided resistance to races 5, 19, 51 and 183. Novel genomic regions for resistance to race 39 were identified on Pv05 and Pv07. Plant resistance genes (R genes) with NB-ARC and LRR domains, which occurred in clusters, were identified as positional candidate genes for genomic regions on Pv02 and Pv04. | |
| dc.identifier.uri | https://dspace.unza.zm/handle/123456789/8900 | |
| dc.language.iso | en | |
| dc.publisher | The University of Zambia | |
| dc.title | Genetic dissection of resistance to anthracnose in yellow bean collection of common beans (Phaseolus vulgaris L.). | |
| dc.type | Thesis |