Cryptosporidiosis is predominantly an urban, anthroponotic infectious disease among Zambian children.
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Date
2021
Authors
Bandaa, Barbara
Siwilab, Joyce
Mukubesac, Andrew N.
Chitangad, Simbarashe
Kaongae, Patrick
Changulaf, Katendi
Simulunduc, Edgar
Saasac, Ngonda
Kelly, Paul
Journal Title
Journal ISSN
Volume Title
Publisher
The University of Zambia
Abstract
Background: Cryptosporidium species are leading causes of diarrhoea in children and immunocompromised
individuals. This study aimed to characterise Cryptosporidium species from children in rural and urban settings
of Zambia.
Methods: Stool samples collected from 490 children aged <5 y with diarrhoea were assessed for Cryptosporidium
oocysts microscopically. A structured questionnaire was used to collect demographic and socioeconomic
characteristics. Positive samples were subjected to PCR and gp60 sequence analysis.
Results: The overall prevalence was 10% (50/490, 95% CI 7.8 to 13.2) with a peak in March, the late rainy season.
Children who came from households where boiling water was not practised (OR=2.5, 95% CI 1.29 to 5.17;
p=0.007) or who had experienced recurrent episodes of diarrhoea (OR=9.31, 95% CI 3.02 to 28.73; p=0.001)
weremore likely to have Cryptosporidium infection. Genotyping of 16 positive samples (14 fromurban and 2 from
rural sources) revealed Cryptosporidium hominis (14/16) and Cryptosporidium parvum (2/16). The Cryptosporidium
hominis subtypes identified were Ia, Ib and Ie with subtype families IeAIIG3 (1), IbA9G3R2 (2), IaA31R3 (3),
IbA9G3 (5), IaA27R3 (1), IaA30R3 (1) and Ia (1). Subtypes IbA9G3 and Ia were identified in children from a rural
area. Cryptosporidium parvum subtypes were IIcA5G3R2 (1) and IIcA5G3a (1).
Conclusions: All isolates successfully genotyped were C. hominis or anthroponotic C. parvum, suggesting that
anthroponotic transmission dominates in Lusaka and the surrounding countryside.
Keywords: children, Cryptosporidium, gp60 gene, risk factors, subtypes, Zambia
Description
Thesis
Keywords
Cryptosporidium species. , Cryptosporidium. , Cryptosporidium--Children. , Diarrhoea diseases--Children.