Studies of fluke infections in Cattle:special reference to the Enzyme-linked Immunosorbent Assay(ELISA)as a diagnostic tool for bovine fascioliasis and shistosomiasis

Thumbnail Image
Ng'ambi, Lily
Journal Title
Journal ISSN
Volume Title
Studies on fluke infections in cattle with special reference to diagnostic methods were carried out. A suitable diagnostic method was necessary for use to investigate the prevalence and distribution of Fasciola spp. and Scliistosoma sj>p. in cattle The Enzyme-linked Immunosorbent Assay (ELISA), based on the antibody-antigen reaction was developed and standardised for sero-diagnosis of fascioliasis and schistose;,nasis in both experimentally and naturally infected cattle. Using crude adult worm antigen preparation, the ELISA test was able to detect antibodies at four weeks post infection in animals experimentally infected with £. hepa_ti_ca and S>. bovis. This is several weeks earlier than infection could be detected by presence of eggs in the faeces. Moreover the ELISA test was specific for either Fasciola or Schistospma_ infections. There were no cross-reacting antibodies between the two parasites, Samples from an abattoir survey which were naturally infected with _F. gjgantica and ^. mat theej_ were examined by the ELISA test sedimentation technique. The ELISA test detected 47 percent as positive, while sedimentation technique 29 percent. No schistosome egges were detected by sedimentation, but the ELISA test detected antibodies in .48 out of564 samples
Fluke Infections---Cattle